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6 grudnia 2018

The Michaelis-Menten expression is commonly used to describe the rate (v) of the enzyme reaction. Enzyme amount was constant. The slope (a) and intercept (b) of the linear curve is then calculated by the least-squares method and [Vmax] and [Km] values can be calculated by the following equations: The Hofstee Plot is shown in Figure 3 and is described by the following equation: where [S]/v is plotted as a function of [S]. It is a very useful number because it can be used to calculate a constant called the turnover number, kp.

You must estimate the absorbance change vs time of your assay mixture, that is the. Enzyme activity is frequently investigated in the medicinal, biochemistry, and food science research fields to elucidate the rate of which reaction occurs and the affinity of the enzyme-substrate interactions. Plot the standard graph of substrate (Adsorption - Concentration). I need to calculate enzyme activity and I dont know how to dit it. This value can be extrapolated from the plateau of the curve on the Michaelis-Menten plot. I have determined the enzyme activity with spectrophotometer at 340nm by the monitoring of NADH oxidation. Krishnendu, first you need to understand the units you are working with. You can also work out activity as nmol/min/mg (then you need to know how much you put in the cuvette let say 1 µg in the 1 mL then meaning that you got 200 nmol/min for 100 µg so you mutliply by 10 to get  2000 nmol/min/mg or 2 µmol/min/mg that is also the enzyme activity. Join Yahoo Answers and get 100 points today. how many grams of C2H3O2Cl will react with O2 to produce 94.6 grams of HCl?

Using the second definition, 293 U = 293 X .926 nmol/min/dl or 271.3 nmol/min/dl. The Lineweaver-Burk, Hofstee, and Eadie plots are expressed as linear plots of the same data derived from the enzyme kinetics reactions. The rate of enzyme reaction is refered to as the velocity of the reaction. The example uses fluorescence but absorbance would work the same way. How can I calculate enzyme velocity from absorbance?